Figure 4.

IFN-γ and IL-2 production by CD38⁺HLA-DR⁺CD8⁺ T cells from patients with glioma is enhanced. (A) Lymphocytes were isolated from patient (n = 11) and healthy donor (n = 10) PBMCs and then cultured for 6 h with ION (1 μg/ml) plus PMA (50 μg/ml). Intracellular cytokine production in CD8⁺ and CD38⁺HLA-DR⁺CD8⁺ T cells was analyzed. Representative images of IFN-γ production by CD8⁺ T cells before and after ION/PMA stimulation. (B) Representative images of IFN-γ production by CD38⁺HLA-DR⁺CD8⁺ T cells. (C) The percentage of IFN-γ production was increased in patient CD8⁺ T cells after ION/PMA stimulation. (D) The production percentage of IFN-γ by CD38⁺HLA-DR⁺CD8⁺ T cells was compared and analyzed before and after ION/PMA stimulation. (E) Representative images of intracellular IL-2 production by CD8⁺ T cells before and after ION/PMA stimulation. (F) Representative images of intracellular IL-2 production by CD38⁺HLA-DR⁺CD8⁺ T cells. (G) The percentage of IL-2 production by CD8⁺ T cells after ION/PMA stimulation. (H) Baseline IL-2 production by CD38⁺HLA-DR⁺CD8⁺ T cells was analyzed and compared after ION/PMA stimulation. CNTL = PBMCs without ION/PMA stimulation. Healthy donor = 10; patient = 11. Values shown are means ± SEM (bars); *p < 0.05 by Student's t-test.