Figure 6.

Co-expression of functional and exhausted molecules in CCR5⁺CD38⁺HLA-DR⁺CD8⁺ T cells in response to glioma. (A) Representative images of gating CD45RA⁺CCR7⁻ in CCR5⁺CD38⁺HLA-DR⁺CD8⁺ T cells after 24 h of co-culture with U87 glioma cells. (B) Responsiveness of CCR5⁺CD38⁺HLA-DR⁺CD8⁺ T cells as revealed by memory phenotypes in patients (n = 11) relative to HD (n = 6). (C) Expressions of T-bet, PD-1, and Tim-3 in CCR5⁺CD38⁺HLA-DR⁺CD8⁺ T cells in response to glioma (n = 16). (D) Representative images of gating CD107a, IFN-γ, and Granzyme B in CCR5⁺CD38⁺HLA-DR⁺CD8⁺ T cells after 24 h of co-culture with U87 glioma cells. (E) Expression of CD107a, IFN-γ, and Granzyme B in CCR5⁺CD38⁺HLA-DR⁺CD8⁺ T cells in response to glioma (n = 24). Values shown are means ± SEM (bars); *p < 0.05, ^#p < 0.01 by Student's t-test.