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Role of SecYEG in the membrane insertion of NG-WALP and NG-WALP-TolR constructs. (a to o) NG-WALP and NG-WALP-TolR constructs were expressed under depleting and nondepleting conditions in a strain conditional for the essential SecYEG component SecE. Cells were fixed with formaldehyde and analyzed by fluorescence microscopy. mNeonGreen (NG) was used as a cytoplasmic control protein. Bars, 3 μm.
