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NG-WALPs with low-hydrophobicity TMDs can insert spontaneously into protein-free liposomes. Purified NG and NG-WALPs were incubated with protein-free liposomes consisting of 75% PE and 25% PG. Subsequently, samples were subjected to sucrose density gradient centrifugation, and three fractions were taken from top to bottom (1 to 3). Fractions were analyzed by Western blotting using anti-His serum.
