TABLE 1.
NAC species | Number of adherent probiotic cells (CFU/ml) |
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---|---|---|---|---|---|---|---|---|
Coinoculation condition |
Postinoculation condition |
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— | +S. boulardii | +S. cerevisiae | +I. occidentalis | — | →S. boulardii | →S. cerevisiae | →I. occidentalis | |
C. tropicalis | 19,118 ± 596 | 401 ± 82 | 411 ± 85 | 390 ± 87 | 21,335 ± 314 | 826 ± 46 | 445 ± 18 | 498 ± 11 |
C. krusei | 20,680 ± 980 | 550 ± 98 | 441 ± 82 | 346 ± 68 | 15,255 ± 731 | 948 ± 83 | 493 ± 22 | 1,000 ± 54 |
C. parapsilosis | 1,307 ± 203 | 184 ± 40 | 186 ± 37 | 205 ± 17 | 5,504 ± 94 | 439 ± 45 | 462 ± 37 | 480 ± 32 |
C. glabrata | 1,757 ± 168 | 257 ± 81 | 261 ± 28 | 171 ± 41 | 832 ± 33 | 374 ± 21 | 386 ± 11 | 424 ± 21 |
For the coinoculation condition (+), probiotic yeasts and non-albicans Candida strains were coinoculated with monolayers of Caco-2 cells and incubated for 3 h. For the postinoculation condition (→), non-albicans Candida strains were applied on an epithelial layer of Caco-2 cells for 60 min prior to inoculation of probiotic yeasts and incubated for an additional 120 min. Candida chrome agar was used to assess CFU of adhered non-albicans Candida on an epithelial layer of Caco-2 cells. All values are expressed as means ± SD. All the values represent statistical significance at P values of <0.05 in comparison to the results determined for the untreated control group (—).