FIG 1.

Glucan and chitin exposure is dependent on time and environmental pH. Wild-type strain (SC5314) was grown in fresh YPD buffered at pH 4 or pH 6 at 37°C for 8 h to 10 h. Cells were fixed and stained with FITC or TRITC label, and median fluorescence intensity of exposed β-1,3-glucan (A, D, E), exposed chitin (B), and total mannans (C) were quantified by FACS. (D) SC5314 cells were grown overnight in pH 4 or pH 6 medium and then freshly inoculated in YPD medium at pH 4 or 6 and incubated at 37°C. (E) Cultures were then swapped to different fresh medium 2 and 6 h postinoculation. Data represent the means and SEMs from seven biological replicates and analyzed by 2-way ANOVAs with Sidak’s post hoc tests. *, P < 0.05 versus respective control at T₀.