FIG 2.

pH-dependent cell wall remodeling is associated with dimorphic Candida species. Clinical strains were grown in fresh YPD buffered at pH 4 or pH 6 at 37°C for 8 h. Cells were fixed and stained with FITC or TRITC label, and the median fluorescence intensities of exposed β1,3-glucan (A) and exposed chitin (B) were quantified by FACS. (C) Non-albicans Candida species were stained for glucan (left) and chitin (right) exposure over time. Data represent the means and SEMs from three biological replicates and analyzed by 2-way ANOVAs with Sidak’s post hoc tests. *, P < 0.05.