FIG 1.

Schematic representation of the data analysis pipeline. Generated MS/MS spectra were subjected to subsequent database searches against the known S. macrospora protein sequences and a 6-frame translation of the S. macrospora genome as well as an independent de novo peptide sequencing method. Putative novel peptide identifications were clustered, filtered, converted to a genome browser readable format, and analyzed in conjunction with RNA-Seq data. Final curation of the genome annotation was performed manually.