FIG 5.

Validation of a stop-loss editing site in the transcript for the white collar 1 protein. (A) Primary amino acid sequence of the white collar 1 protein. Editing results in the conversion of a stop codon into a tryptophan codon and extends the amino acid sequence by 131 amino acids. As a consequence, the protein carries a C-terminal histone deacetylase domain (HDAC). Peptides encoded by the canonical gene are shown in red, while the blue peptide is unique to the novel C-terminal sequence. (B) Annotated MS/MS spectrum of the editing-specific peptide observed in the 7-day data set. (C) Domain structure of the white collar 1 protein, including the HDAC domain (green box) in the extended C terminus (blue box). PAS, Per-Arnt-Sim domain; GATA, GATA-type zinc finger transcription factor domain.