TABLE 2.

Classification of identified SAAVs by type^a

SAAVb	Mass shift (Da)	No. of observations	Edited and nonedited detected (%)	Median PROVEAN score	Predicted deleterious (%)
K→E	0.94763	32	53	−1.2565	21.9
I→V	−14.01565	19	95	−0.689	0
S→G	−30.01057	14	100	−1.8475	28.6
R→G	−99.07965	11	90	−3.349	63.6
K→R	28.00615	10	70	−1.1875	10.0
T→A	−30.01057	7	100	−0.792	42.9
Q→R	28.04253	6	100	−0.797	16.7
Y→C	−60.05414	5	100	−6.16	80.0
M→V	−31.97208	5	75	−1.047	20.0
E→G	−72.02113	2	100	−2.833	50.0
N→S	−27.0109	1	100	−2.053	0
I→M	17.95643	1	100	−2.867	100
Total		113	81	−1.220	26.8

^aTotal number of observed single-amino-acid variation (SAAV) putatively caused by mRNA editing events in the 7-day sample. Respective theoretical mass shifts of each amino acid exchange are given. Additionally, percentages of sites found for both the edited as well as the nonedited variant are given. PROVEAN prediction score was retrieved for each individual SAAV via the PROVEAN web interface (70), and the median score for each class of SAAV was calculated. A default threshold of −2.5 was used to estimate the extent of potentially deleterious variants.

^bN→D events were not considered, as they can be caused by RNA editing as well as by asparagine deamidation on the protein/peptide level.