Figure 3.
Therapeutic immunization with nanofibers significantly suppressed the growth of established TC-1 with a diameter of 2–3 mm.
Notes: (A) The experimental protocol. (B) Left: The tumor volumes were monitored every 3 days. Right: The percentage of tumor-free mice was calculated on the indicated days. The differences were determined using one-way analysis of variance (ANOVA) followed by Tukey’s multiple comparisons test. *P < 0.05; ***P < 0.001; n = 6. (C) Left: representative pictures of tumor masses; Middle: weight of tumor masses; Right: spleen weight. *p < 0.05; ***p < 0.001; n = 4. (D) E744-62 specific IFN-γ-expressing lymphocytes were detected by ELISPOT; Left: representative pictures; Right: statistical data. * p < 0.05; ns: p ≥ 0.05; n = 4.
Abbreviations: s.c., subcutaneous; IFN-γ, interferon-γ; ELISPOT, enzyme-linked immunospot assay.