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. 2019 Oct 10;14:8209–8219. doi: 10.2147/IJN.S214525

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© 2019 Li et al.

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Immunization with E7_44-62-Q11 nanofibers significantly induced an E7_44-62-specific CTL response.

Notes: C57BL/6 mice were immunization with either E7_44-62 peptide, the mixture of E7_44-62 and Q11 nanofibers, or E7_44-62-Q11 nanofibers, and administered with PBS or Q11 nanofibers as the controls. In addition, the mice were intravenously injected with CFSE^high and CFSE^low splenocytes 7 days after the last immunization. (A) The representative flow cytometry histograms for CFSE-labelled cells isolated from spleens and lymph nodes 15 hrs after injection. (B) Statistical data of E7-specific lysis of CFSE-labelled splenocytes. Left: spleens; Right: lymph nodes. The differences were determined using unpaired Student’s t-test. *P < 0.05; **P < 0.01; n = 4.

Abbreviations: CFSE, carboxyfluorescein succinimidyl ester; LN, inguinal lymph node.