Figure 9.

CNTF-activated astrocytes are more resistant to glycolysis inhibition. In Veh-treated cultures, exposure to 0.4 mm IAA for 10 min triggers a massive degeneration of GFAP-positive astrocytes (green) and MAP2-positive neurons (red) at 24 h compared with control conditions. In CNTF-treated cultures, the rate of survival is markedly improved for astrocytes, and neuronal dendrites are less dystrophic. In the presence of 3 mm 4-CIN (IAA plus 4-CIN), CNTF looses its protective effects, and both neurons and astrocytes display a pathological morphology and a low survival rate. Images are representative of results obtained in two to three wells from three to six independent cultures. Scale bar, 50 μm.