Figure 3.

Exogenous expression of APP(V642I) or APP–BP1 in neurons causes increases of Rab5 and EEA1 protein levels but not of Rab11 levels. Primary neurons were infected with HSV vectors expressing APP–BP1, APP(V642I), or LacZ; 16 h later, the cells were lysed. Equal amounts of protein were separated by SDS-PAGE on three separate gels, and each was transferred to a polyvinylidene difluoride (PVDF) membrane. Blots were probed with rabbit polyclonal anti-Rab5, rabbit polyclonal anti-EEA1, or mouse monoclonal anti-Rab11. Each blot was also probed with mouse monoclonal anti-GAPDH to standardize for loading differences between samples. ANOVA revealed that neurons expressing APP–BP1 or APP(V642I) presented a significant increase in Rab5 and EEA1 protein levels, but not in Rab11 levels, compared with mock- or HSV–LacZ-infected control neurons. *p < 0.01. Values shown are densitometry values standardized to GAPDH. Insets show a representative gel for each condition.