Figure 1.

Biolistic transfection of GFP and Cre into a single cortical neuron. A, A schematic drawing showing that transfection of Cre– nuclear localized signal (NLS) induces a deletion of BDNF gene from neurons of floxed BDNF transgenic mice. Transfected neurons were marked with GFP fluorescence. B, An image of cortical slice, in which somas of many neurons were visualized in blue by fluorescent Nissl staining. Scale bar: (in B) B–D, 10 μm. C, An image of a transfected neuron that expresses GFP (green) in the soma and dendrites and Cre (yellow) in the nucleus. D, A superimposed image of B and C.