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. 2007 Jul 4;27(27):7234–7244. doi: 10.1523/JNEUROSCI.1943-07.2007

Figure 2.

Figure 2.

Absence of BDNF mRNA and deletion of BDNF protein in a GFP-positive cell. A, Fluorescent image of in situ hybridization of GFP mRNA. The arrow indicates a transfected neuron that expressed green signal. Scale bar: B–D, 10 μm. B, Image of in situ hybridization of BDNF mRNA. The arrow indicates that the cell that expressed GFP mRNA in A does not have BDNF mRNA. C, Superposed image of A and B. Note the deletion of BDNF mRNA in the cell with GFP mRNA. D, Control image of in situ hybridization by BDNF sense probe. E, Fluorescent image of Nissl staining (blue) of cortical slices. Scale bar: F–H, 10 μm. F, Fluorescent image of GFP-positive neuron (green), which was immunohistochemically stained with anti-GFP antibody. G, Fluorescent image of BDNF (red). Arrows indicate a GFP-positive transfected neuron. H, Superposed image of GFP and BDNF.