Figure 7.
Increase in the number of GABAergic synaptic terminals with days in vitro. The mean numbers of GAD65-positive puncta around the soma of neurons in the single confocal section are plotted at days in vitro indicated at the abscissa. The mean ± SEM values at 6, 9, 12, and 15 DIV are 2.1 ± 0.3, 4.1 ± 0.4, 6.2 ± 0.4, and 7.6 ± 0.5, respectively. The numbers of cells at each group are shown in parentheses.