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. 2007 Jul 4;27(27):7284–7296. doi: 10.1523/JNEUROSCI.0731-07.2007

Figure 1.

Figure 1.

Syntabulin and KIF5B associate with immunoisolated AZ precursors containing Bassoon and syntaxin-1. A, B, Bassoon- or syntabulin-associated membranous organelles were immunoisolated from light membrane fractions of fetal rat brain with Dyna magnetic beads coated with either anti-Bassoon (A) or anti-syntabulin (B) antibody, or normal IgG as controls. The bead-bound membranous organelles were solubilized and resolved by 3–8% Tris-acetate PAGE (top three panels) or 4–12% Bis-Tris PAGE (other panels) and sequentially detected by Western blot with antibodies as indicated in the same membranes after stripping between each application of antibody. Relative purity of the isolated Bassoon- or syntabulin-associated membranous vesicles was assessed by immunoblotting the AZ components (Bassoon, Piccolo, N-cadherin, syntaxin-1, SNAP-25, KIF5B, or β-catenin) and the markers for other membranous organelles including EEA1 (endosomes), p115 (Golgi), and synaptophysin (SVs).