Figure 6.
The ATP-independent component of NMDA receptor-dependent respiratory stimulation. A, Glutamate addition simultaneous with 0.2 μg/ml oligomycin (G+O); the numbers correspond to the images shown in B. B, Simultaneous imaging of TMRM+ and Fluo-5F fluorescence at the time points shown in A. Note the extensive deregulation of the cells after glutamate in the presence of oligomycin (i.e., high [Ca2+]c, low membrane potentials). C, Oligomycin addition (O) before glutamate. Note the respiratory inhibition with oligomycin showing the residual proton leak and the small stimulation with subsequent glutamate.