Figure 9.

Phospho-ERK1/2 elevations after NE treatment are attenuated in the presence of prazosin or U10126. Single-plane confocal images show tissue from slices receiving only NE treatment (100 μm) (A–C) or treated with 100 μm NE in the presence of either 10 μm prazosin (D–F) or 10 μm U0126 (G–I). Each row shows three images of an identical field within the parvicellular PVH at 100× magnification, sorted according to color channel: CRH (A, D, G), phospho-ERK1/2 (B, E, H), or the overlay of these channels (C, F, I). Neurons with both CRH and phospho-ERK1/2 immunoreactivities appear yellow in C (marked by arrowheads); this color is not evident under conditions in which prazosin or U0126 are used (E, F; H, I). Scale bar, 20 μm (applies to all panels). Readers preferring to view these data in pseudocolored form may do so by referring to supplemental Figure S6 (available at www.jneurosci.org as supplemental material).