Fig. 4.
PH overcomes Sor resistance in HepG2SR and Huh7SR cells in vitro. a PH inhibited cell growth in Sor resistant HepG2SR and Huh7SR cells. Cell viability was assessed by MTT assay. b PH enhanced DNA fragmentation in SR-HCCs. c The protein levels of caspase-3, caspase-9 and PARP after exposing SR-HCCs to PH. d PH inhibited STAT3, ERK, AKT/mTOR signaling in HCC cells. e pSTAT-3 activity was measured by ELISA. f SHP-1 phosphatase activity in HepG2SR and Huh7SR. g SHP-1 mRNA levels were analyzed by qPCR. h SR-HCC cells were transfected with control siRNA or SHP-1 siRNA for 48 h there exposed to PH and subjected to Western blot assay of p-STAT3 and SHP-1 or seeded on a 6-well plate for the colony forming assay. Experiments were conducted in triplicate and mean values ± SD (bars) are shown. *p < 0.05, and **p < 0.01 compared to control