Fig. 3.

Reactive oxygen species (ROS) production in isolated mature adipocytes. Reactive oxygen species (ROS) production was determined in adipose cells isolated in pigs selected for low (LRFI) or high (HRFI) RFI and housed in good or poor hygiene conditions during 6 weeks. A fluorimetric assay using 2,7-dihydrochlorofluorescein (DHCF) was used to estimate ROS production into cells. Adipocytes were cultured in media with or without bacterial lipopolysaccharides (LPS: 1 mg/mL) or tumor necrosis factor (TNF-alpha: 1 ng/mL). A fluorimetric assay using 2,7-dihydrochlorofluorescein (DHCF) was used to estimate ROS production into cells. Data are expressed as mean ± S.E.M. (n = 8 pigs per group). LxH: interaction effect between genetic line (L) and hygiene conditions (H); LPS: effect of LPS; TNF-alpha: effect of TNF-alpha. **P < 0.01. Hatched charts are used for poor hygiene conditions, and full charts are used for good hygiene conditions; red color is used for high RFI pigs and blue color is used for low RFI pigs