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. 2019 Oct 16;7:259. doi: 10.1186/s40425-019-0739-1

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Histones are involved in CB-NK anti-MM activity. a. 3 h cytotoxicity assays comparing CB-NK control (CB-NK siRNA ctrl) vs CB-NK with knockdown of H2AZ (CB-NK siRNA H2AZ). b. 3 h cytotoxicity assays comparing CB-NK control (CB-NK GFP) vs CB-NK over-expressing H2AZ (CB-NK H2AZ). c. H2AZ levels in peripheral blood NK cells (PB-NK) vs CB-NK, analyzed by confocal fluorescence microscopy. Representative image of H2AZ levels is shown on the right. d. 3 h cytotoxicity assays comparing PB-NK control (PB-NK siRNA ctrl) vs PB-NK with knockdown of H2AZ (PB-NK siRNA H2AZ). e. 3 h cytotoxicity assays comparing CB-NK (CB-NK siRNA ctrl) with CB-NK where histones H2AZ, H4 and H1.5 were knockdown. Assays were performed at least in three independent experiments. a: all groups analyzed compared to CB-NK siRNA ctrl are different (p < 0.05). b: at least one group analyzed compared to CB-NK siRNA ctrl is different (p < 0.05). *p < 0.05. ** p < 0.001. Efficiency of knockdown of H2AZ was confirmed by Western Blot and by flow cytometry (Additional file 1: Figure S3)