Fig. 4.

CB-NK and histones promote pyroptosis with in vivo MM cell death and concomitant inflammation. a. 3 h cytotoxicity assays of CB-NK against MM and non-MM K562 cells, adding Caspase-1 inhibitor to analyze the impact on pyroptotic cell death. b. Impact of recombinant H2AZ on viability of MM and non-MM K562 cells. HSA: Human Serum Albumin (2 μM) was added as protein control in parallel with H2AZ (2 μM). Y-VAD was added to analyze the impact on H2AZ effect. Cell proliferation was measured by viable cell count. c to g: Anti-MM and pro-inflammatory in vivo activity of H2AZ. NSG mice received ARP1 cells and were treated with recombinant H2AZ. Weekly bioluminescence (c and d) images and kappa ELISA light chains measurements (e) were performed. f. Lung and tissues of mice untreated (MM) or treated with H2AZ (MM + H2AZ). g: CD138 expression in MM cells of mice tissues. *p < 0.05