Fig. 7.

Released NK cell histones also promote T cell/MM cell clustering increasing T cell anti-MM activity: a. CB-NK and MM cells were co-cultured and the SIPs were collected and added into 3 h cytotoxicity assays against MM cells adding as effectors T cells (b). SIPs were added in parallel to U266 cells alone as control, and CB-NK were also included as effectors to compare the efficacy. c-f: Impact of histones in the SIPs capacity to increase T cell anti-tumor activity. CB-NK ctrl (siRNA ctrl) and CB-NK with knockdown of histones (siRNA Hist) were co-cultured with MM cells to obtain both SIPs siRNA ctrl and SIPs siRNA Hist (c). These SIPs were added into T-cell/tumor cell co-cultures, and the impact on cell clustering formation after 4 h (d-e) and on T cell cytotoxicity after 48 h was analyzed (f). g-k: Impact of recombinant H2AZ (g-i) and H4 (j and k), in the formation of cell clustering after 4 h, and in the T cell cytotoxicity vs MM and non-MM K562 cells after 48 h. Heparin was added in parallel. *p < 0.05. ** p < 0.001