Figure 3.

dTau abrogation affects long term memory. A, B, tau^KO and tau^MI mutants present enhanced LTM (*p < 0.0001), whereas 3 min memory and ARM are not affected (p > 0.05). The number of experimental replicates (n) is indicated within the bars. C, CXM administration eliminated the enhanced LTM of tau^MI flies (*p < 0.0001). D, Representative Western blot of head lysates from flies expressing UAS-dtauRNAi with Elav-Gal4 using an anti-dTau antibody. The genotype of control animals was Elav-Gal4/+. Compared with its levels in control animals, dTau was significantly reduced. For the quantification, tau levels were normalized using the syntaxin (Syx) loading control and shown as a ratio of their mean ± SEM values relative to its respective levels in control flies, which was set to 1. The star indicates significant differences from the control indicative of reduced dTau levels (*p < 0.0001). E, Three minute memory is not affected after the downregulation of dTau in the adult MBs using dnc compared with driver and transgene heterozygotes. F, Enhanced LTM performance upon abrogation of dTau during adulthood using Elav (*p < 0.0001), LeoMB (*p = 0.0027), MB247 (*p = 0.0199), dnc (*p = 0.0001), c305α (*p = 0.00001), and c739 (*p = 0.0005).