Table 1.
A role of RGS4 in CFA-induced gene upregulation
| Gene | Description | RGS4WT day 18 post-CFA | RGS4KO day 18 post-CFA | Interaction padj |
|---|---|---|---|---|
| Ghsr | Growth hormone secretagogue receptor | 0.96 | −0.12 | 2.63E-08 |
| Cbln1 | Cerebellin 1 precursor protein | 0.58 | −0.34 | 5.10E-07 |
| Hcn1 | Hyperpolarization-activated, cyclic nucleotide-gated K + 1 | 0.47 | −0.02 | 2.11E-05 |
| Prss12 | Protease, serine 12 neurotrypsin (motopsin) | 0.55 | −0.13 | 2.86E-05 |
| Pcdh9 | Protocadherin 9 | 0.34 | −0.10 | 0.0001 |
| Gm13339 | Predicted gene 13339 | 0.17 | −0.24 | 0.0003 |
| Ndst4 | N-Deacetylase/N-sulfotransferase (heparin glucosaminyl) 4 | 0.39 | −0.11 | 0.0009 |
| Slit3 | Slit homolog 3 | 0.41 | −0.07 | 0.0010 |
| Nefl | Neurofilament, light polypeptide | 0.40 | −0.09 | 0.0012 |
| Caln1 | Calneuron 1 | 0.33 | −0.03 | 0.0045 |
| Sspo | SCO-spondin | 1.52 | −0.90 | 0.0045 |
| Cela1 | Chymotrypsin-like elastase family, member 1 | 0.91 | −0.66 | 0.0045 |
| Lrrc38 | Leucine-rich repeat containing 38 | 0.88 | −0.18 | 0.0049 |
| Esrrb | Estrogen-related receptor, β | 1.07 | −0.32 | 0.0053 |
| Igf1 | Insulin-like growth factor 1 | 0.53 | −0.19 | 0.0053 |
| Gm11413 | Predicted gene 11413 | 1.00 | −0.41 | 0.0053 |
| Nr4a2 | Nuclear receptor subfamily 4, group A, member 2 | 0.56 | −0.49 | 0.0053 |
| Gm10222 | Predicted gene 10222 | 0.07 | −0.25 | 0.0055 |
| Sim1 | Single-minded homolog 1 | 0.84 | −0.13 | 0.0062 |
| Krt222 | Keratin 222 | 0.21 | −0.10 | 0.0064 |
| Anxa4 | Annexin A4 | 0.86 | −0.24 | 0.0067 |
| Ntng1 | Netrin G1 | 0.21 | −0.07 | 0.0067 |
| Tfrc | Transferrin receptor | 0.07 | −0.24 | 0.0069 |
| Ugcg | UDP-glucose ceramide glucosyltransferase | 0.05 | −0.12 | 0.0070 |
| Mfap3l | Microfibrillar-associated protein 3-like | 0.25 | −0.14 | 0.0104 |
| Postn | Periostin, osteoblast-specific factor | 2.20 | −0.16 | 0.0111 |
| Gm13340 | Predicted gene 13340 | 0.13 | −0.11 | 0.0123 |
| Calcb | Calcitonin-related polypeptide, β | 2.29 | −0.01 | 0.0131 |
| Scrg1 | Scrapie responsive gene 1 | 0.42 | −0.09 | 0.0131 |
| 4632404H12Rik | RIKEN cDNA 4632404H12 gene | 0.30 | −0.12 | 0.0131 |
| Pax6 | Paired box 6 | 0.82 | −0.44 | 0.0131 |
| Cpox | Coproporphyrinogen oxidase | 0.16 | −0.25 | 0.0131 |
| Slc35a5 | Solute carrier family 35, member A5 | 0.10 | −0.19 | 0.0131 |
| Gm11408 | Predicted gene 11408 | 0.07 | −0.27 | 0.0131 |
| Cartpt | CART prepropeptide | 3.06 | −0.10 | 0.0134 |
| Paqr8 | Progestin and adipoQ receptor family member VIII | 0.36 | −0.10 | 0.0171 |
| Gfod1 | Glucose-fructose oxidoreductase domain containing 1 | 0.40 | −0.06 | 0.0171 |
| Cep97 | Centrosomal protein 97 | 0.11 | −0.37 | 0.0171 |
| Dner | Delta/notch-like EGF repeat containing | 0.14 | −0.05 | 0.0182 |
| Kcng4 | Potassium voltage-gated channel, subfamily G, member 4 | 1.09 | −0.17 | 0.0183 |
| Adamtsl5 | ADAMTS-like 5 | 0.67 | −0.20 | 0.0197 |
| Pappa | Pregnancy-associated plasma protein A | 0.55 | −0.06 | 0.0204 |
| Abhd10 | Abhydrolase domain containing 10 | 0.11 | −0.22 | 0.0225 |
| Ppm1e | Protein phosphatase 1E (PP2C domain containing) | 0.28 | −0.03 | 0.0244 |
| Mbnl2 | Muscleblind-like splicing factor 2 | 0.16 | −0.04 | 0.0266 |
| Kcnn1 | Potassium intermediate/small conductance calcium-activated channel, subfamily N, member 1 | 0.26 | −0.13 | 0.0266 |
| Mbnl1 | Muscleblind-like splicing factor 1 | 0.10 | −0.07 | 0.0266 |
| Nell1 | NEL-like 1 | 0.13 | −0.09 | 0.0267 |
| Spp1 | Secreted phosphoprotein 1 | 1.41 | −0.26 | 0.0292 |
| Ube2g1 | Ubiquitin-conjugating enzyme E2G 1 | 0.12 | −0.14 | 0.0301 |
| Chodl | Chondrolectin | 1.45 | −0.23 | 0.0305 |
| 1700020I14Rik | RIKEN cDNA 1700020I14 gene | 0.32 | −0.06 | 0.0306 |
| Heatr5b | HEAT repeat containing 5B | 0.17 | −0.01 | 0.0308 |
| Ppargc1a | Peroxisome proliferative activated receptor, gamma, coactivator 1 α | 0.29 | −0.12 | 0.0308 |
| Fblim1 | Filamin binding LIM protein 1 | 0.83 | −0.34 | 0.0321 |
| Grin3b | Glutamate receptor, ionotropic, NMDA3B | 0.90 | −0.19 | 0.0336 |
| Nrip3 | Nuclear receptor-interacting protein 3 | 0.24 | −0.06 | 0.0404 |
| 9330132A10Rik | RIKEN cDNA 9330132A10 gene | 0.22 | −0.07 | 0.0404 |
| Spp2 | Secreted phosphoprotein 2 | 1.71 | −0.70 | 0.0404 |
| Vdr | Vitamin D (1,25-dihydroxyvitamin D3) receptor | 2.43 | −0.12 | 0.0479 |
| Eif5a2 | Eukaryotic translation initiation factor 5A2 | 0.23 | −0.08 | 0.0490 |
| Inpp5j | Inositol polyphosphate 5-phosphatase J | 0.39 | −0.07 | 0.0496 |
Multifactor analysis reveals that a set of 62 genes are upregulated by CFA treatment in the RGS4WT group, whereas these genes are downregulated in the RGS4KO group. Table 1 contains genes with interaction-adjusted p < 0.05.