Figure 5.

Down-regulation of PBF inhibits the AKT/mTOR and Wnt/β-catenin signaling pathways. (A) and (B) AKT/mTOR pathway including AKT, p-AKT, mTOR, p-mTOR and p-p70S6K was detected by western blot and quantified in density by ImageJ software; (C) and (D) Wnt/β-catenin pathway including Wnt3a, β-catenin, E-cad, N-cad, Vimentin, Snail1 and Snail2 was detected by western blot and quantified in density by ImageJ software. All experiments were repeated 3 times. *P represented significant difference.