TABLE 2.

qPCR primer sequences

Gene	Forward	Reverse
B4GALT6	ACA TTA TAC CTC GAG CTT GTA CC	CTT CTC CCT CTC TTC GTC CT
CERT	CCT GAG CAC GAA GAT ACC AAA	TGA AGA TGA AAC AGA GTA TGG CT
FA2H	GTA CTC GAT GAG GCT CCA GA	GCA ACG TCC GAC TCT TCA
GNB2L1	TGG TCT TCA GCT TGC AGT TAG	GCA AAT ACA CTG TCC AGG ATG
HPRT1	GTA TTC ATT ATA GTC AAG GGC ATA TCC	AGA TGG TCA AGG TCG CAA
SGMS1	GTA CAG ATA GTC CCC ACA CAT	GCA TTT CAA CTG TTC TCC GAA G
SGMS2	GTG TGC TAC AAG AAT GCA GAT G	CTT CCT CTT CAG CGG TCA C
SGPL1	ATA TGA GTT TGT CTT CCA GCC A	CTT GGT CTT GTT CAA CTT GTC TTG
SMPD1	GAG AGA GAT GAG GCG GAG A	CTG GCT CTA TGA AGC GAT GG
SMPD3	GGT CCT GAG GTG TGC TTC	TCT TTG CCA GCC GCT AC
SPHK1	TTC ACG CTG ATG CTC ACT G	CCG TTC ACC ACC TCG TG
SPHK2	CCT TCA ACC TCA TCC AGA CAG	CCC GTT CAG CAC CTC AT
RPLP0	TGT CTG CTC CCA CAA TGA AAC	TCG TCT TTA AAC CCT GCG TG
UGCG	GCA TTG CAA CTT GAG TGG AC	GAT GTG TTG GAT CAA GCA GGA
UGT8	AAG ACA CCA AGA CAA AGC CA	GAA TTC CCA AGA CCC ACT CTG

GNB2L1, G protein subunit beta 2; HPRT1, hypoxanthine phosphoribosyltransferase 1; RPLP0, ribosomal protein lateral stalk subunit P0; UGCG, UDP-glucose ceramide glucosyltransferase.