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. 2019 Nov;25(11):1549–1560. doi: 10.1261/rna.071811.119

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© 2019 Musalgaonkar et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 2. — Nmd3 binds to subunits lacking Rpl1. (A), (B) Polysome profiles and western blots for monitoring sedimentation of Nmd3, Rpl1, and Rpl8 from extracts of WT (KBM13) and GAL::RPL1 (KBM20) cells, respectively, grown in galactose media followed by 2 h growth after adding glucose. (C) Western blots for affinity purification of Nmd3-TAP (AJY4009, lanes 1,2) and Arx1-TAP (AJY4013, lanes 3,4) from GAL::RPL1 cells either grown in galactose medium continually (lanes 1,3) or for 2 h after addition of glucose (lanes 2,4). (D) Ratios of Rpl1 to Rpl8 signal from western blot in C were calculated and normalized to the Rpl1 to Rpl8 ratios for cells grown continuously in galactose medium.