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. 2019 Nov;25(11):1549–1560. doi: 10.1261/rna.071811.119

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© 2019 Musalgaonkar et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 5. — High copy expression of Mex67–Mtr2 heterodimer specifically suppresses the 60S export defect caused by Rpl1 loss. Rpl25–GFP viewed in (A) WT (KBM13) transformed with RPL25–GFP (pAJ907) and empty vector (pRS426) and in rpl1bΔ P_GAL-RPL1A (KBM20) with RPL25–GFP and empty vector or (B) high copy MEX67+MTR2 (pAJ3972), ARX1 (pAJ4315), or BUD20 (pAJ4316) and grown in Leu-Ura-media with galactose for 48 h and then diluted fivefold in glucose-containing media and grown for 1.5 h more. (C) Tenfold serial dilutions of the KBM13 or KBM20 transformed with empty vector or MEX67+MTR2 were spotted on glucose-containing selective media to repress P_GAL:RPL1A in KBM20. (D) Rpl25–GFP viewed in the upper panel: rpl1bΔ (KBM17) transformed with RPL25–GFP (pAJ907) and empty vector (pRS426) and lower panel: KBM17 transformed with RPL25–GFP and MEX67 MTR2 and grown in Leu-Ura-media with glucose for 48 h and then diluted fivefold in glucose-containing media and grown for 1.5 h more.