Figure 5.
Cell cycle analysis via flow cytometric analysis. Significant induction of a G1-phase cell cycle arrest in MPC cells by BYL719, everolimus, or sunitinib alone and (even stronger) by the BYL719/everolimus and sunitinib/everolimus combination treatments at 24 h of treatment. Significant induction of a G1-phase cell cycle arrest in MTT cells by everolimus (10 nM) and sunitinib (1 µM/2 µM) and (even stronger) by the BYL719/everolimus and sunitinib/everolimus combination treatment at 24 h of treatment. The arithmetic means and SD of at least three independent experiments are shown. *Statistically significant differences in comparison with the DMSO control (P < 0.05).