Figure 1.
Bone cancer induces behavioral hypersensitivity and bone destruction. A, B, Intratibia inoculation with Walker 256 mammary gland carcinoma cells (4 × 105) induces significant mechanical allodynia (A) and thermal hyperalgesia (B) in the ipsilateral hindpaw. C, Intratibia inoculation with Walker 256 mammary gland carcinoma cells induces spontaneous flinches in the hindpaw ipsilateral to the affected limb. The number of times the hindpaw was counted per 5 min during a 10 min observation period, and the average number of flinches per 5 min were calculated. *p < 0.05; **p < 0.01 versus sham rats. D, Radiographs represent robust radiolucent lesions of the tibia ipsilateral to the tumor-bearing limb on PTDs 14 and 21. Contr., Contralateral; Ipsi., ipsilateral.