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. 2015 May 20;35(20):7950–7963. doi: 10.1523/JNEUROSCI.5250-14.2015

Figure 2.

Figure 2.

Time course of astrocytic and microglial activation on the lumbar spinal cord following bone cancer. A, Western blot analysis reveals an early upregulation of GFAP from PTD 7 and a delayed upregulation of IBA-1 from PTD 14 in the ipsilateral L3–L5 spinal dorsal horn. B–D, Immunohistochemisty data show astrogliosis, as indicated by intense GFAP immunoreactivity and hypertrophied astrocytes with thick processes, and microgliosis, as indicated by intense IBA-1 immunoreactivity and large cell bodies and short or thick processes of microglia in the ipsilateral spinal dorsal horn. Astrogliosis and microgliosis occurred from early phase (PTD 7) and advanced phase (PTD 14), respectively. *p < 0.05; **p < 0.01 versus sham rats.