Figure 11.

Anatomical description of PMPV neurons. Dense arborizations of PMPV cluster neurons were visualized by differential fluorescence reporter expression using UAS:Flybow 2.0/m-hs-FLP; R66A09-Gal4/Trh-FIF. Random expression of GFP and mCitrine in 5-HT neurons covered by R66A09-Gal4 allows for distinguishing the arborizations of two neurons, PMPV1 (A) and PMPV2 (B). Left images represent GFP (green), mCitrine (yellow) expression and anti-DLG immunoreactivity (blue). The center images show GFP and mCitrine expression overlapping with anti-5-HT immunoreactivity (red). 3D reconstructions of the two neurons are shown on the right, without (top) and with anti-DLG staining (bottom). Scale bars, 50 μm.