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. 2015 Sep 16;35(37):12792–12812. doi: 10.1523/JNEUROSCI.1638-15.2015

Figure 2.

Figure 2.

Behavioral quiescence is induced by 5-HT neurons in the brain. A, Expression of dTRPA1-mCherry under control of Trh-Gal4 in the brain, the thoracic, and the abdominal ganglia (left). Gene expression is restricted to brain neurons using Tsh-Gal80 (right). Magenta represents anti-RFP immunoreactivity against dTRPA1-mCherry. Green represents anti-5-HT immunoreactivity. White represents the overlap. AB, Abdominal ganglia; MS, mesothoracic ganglia; MT, metathoracic ganglia; PR, prothoracic ganglia. Scale bars, 50 μm. B, Thermogenetic activation of all Trh-Gal4-positive neurons and of Trh-Gal4-positive neurons in the brain only equally decreases locomotor activity. Bars indicate mean ± SEM (n = 27–28). n.s., Not significant (p > 0.05). ***p < 0.001.