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. 2015 Sep 16;35(37):12792–12812. doi: 10.1523/JNEUROSCI.1638-15.2015

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Copyright © 2015 the authors 0270-6474/15/3512792-21$15.00/0

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Figure 9. — Restriction of gene expression to confined 5-HT neurons using intersectional genetics. Expression of mCD8:GFP (anti-GFP immunoreactivity) in the brain under control of different Gal4 driver lines using the UAS-Gal4 system (left column) and restriction of mCD8:GFP expression to defined 5-HT neurons (middle and right columns). Middle column represents anti-GFP immunostainings. Right column represents the overlap of anti-GFP staining (green) and anti-5-HT immunoreactivity (magenta). The identities and positions of 5-HT neuron clusters expressing mCD8:GFP are indicated by red arrows and dashed circles. Scale bars, 50 μm.