Table 2.
Quantification of serotoninergic and nonserotoninergic neurons in the central brain expressing dTRPA1-mCherry under control of three different Trh-Gal4 linesa
| 5-HT cell cluster | 5-HT-positive | Trh-Gal4 (1) (Park et al., 2006) | Trh-Gal4 (2) (Sitaraman et al., 2012) | Trh-Gal4 (3) (Alekseyenko et al., 2010) |
|---|---|---|---|---|
| ALP | 6 ± 0 | 2 ± 0 | 4 ± 1 | 4 ± 1 |
| AMP | 2 ± 0 | 0 ± 0 | 2 ± 0 | 2 ± 0 |
| ADMP | 2 ± 0 | 0 ± 0 | 2 ± 0 | 2 ± 0 |
| LP | 24 ± 3 | 11 ± 1 | 19 ± 3 | 18 ± 3 |
| SEL | 10 ± 2 | 5 ± 1 | 12 ± 0 | 11 ± 1 |
| SEM | 10 ± 3 | 8 ± 2 | 8 ± 3 | 9 ± 3 |
| PLP | 4 ± 0 | 4 ± 0 | 4 ± 0 | 4 ± 0 |
| PMPD | 6 ± 0 | 4 ± 0 | 6 ± 0 | 6 ± 0 |
| PMPM | 13 ± 2 | 5 ± 2 | 10 ± 3 | 12 ± 3 |
| PMPV | 14 ± 3 | 9 ± 3 | 10 ± 2 | 8 ± 3 |
| Σ 5-HT neurons | 91 ± 13 | 48 ± 9 | 79 ± 12 | 76 ± 14 |
| Non-5-HT neurons | 233 ± 24 | 168 ± 12 | 198 ± 22 |
aThe number of serotoninergic (5-HT-positive) neurons and nonserotoninergic cells in each identified neuron cluster of the central brain (i.e., excluding the optical lobes) covered by Trh-Gal4 (1), Trh-Gal4 (2), and Trh-Gal4 (3) are indicated as mean ± SD (n = 3 brains, both hemispheres). The references indicate the description of the respective Gal4 lines.