Table 5.
Somata cluster designation of 5-HT neurons covered by different Gal4 driver lines and the behavioral consequence of thermogenetic activation of these neuronsa
| Gal4 lines | PMPV big | PMPV small | PMPD | SEM | SEL | PLP | Behavioral effect |
|---|---|---|---|---|---|---|---|
| R66A09 | + | + | + | + | − | − | + |
| R67B05 | + | + | + | + | + | + | + |
| R70A11 | + | − | − | − | − | − | − |
| R23E12 | − | − | + | − | − | − | − |
| R75D10 | − | − | − | − | − | + | − |
| R65D03 | − | − | − | + | + | + | − |
| R35C08 | − | − | − | − | + | − | − |
aRows indicate the identity of neuronal clusters in which mCherry-dTRPA1 was expressed using different Gal4-driver strains. +, Thermogenetic activation of the indicated neurons caused a significant reduction in locomotion activity; −, thermogenetic activation of the indicated neurons did not alter locomotion activity. The combinatorial analysis reveals that locomotion activity is affected by neurons of the PMPV cluster with small somata.