Figure 10.

DHA promotes neurite growth through the PTEN/pAKT/mTOR signaling pathway by miR-21 modulation. A–G, In embryonic cortical neuron cultures, 3 μm DHA significantly enhances the neurite growth with concomitant reduction in PTEN and increase in pAKT level. H, After rapamycin treatment, no significant neurite outgrowth was observed after incubation with DHA. I, J, In cortical neurons transfected with miR-21 inhibitor, the ability to suppress PTEN expression and promote neurite growth by DHA was attenuated. *p < 0.05, **p < 0.01. ns, Not significant.