Figure 11.

DHA downregulates PTEN expression and upregulates p-AKT expression in vitro. The expression levels of PTEN and pAKT in cortical neurons were measured by Western blot. The qualitative expression of PTEN and pAKT is shown (B, D). A, B, Quantitative analysis of PTEN protein levels revealed a significant decrease after DHA treatment. C, D, Quantitative analysis of pAKT protein levels revealed a significant increase after DHA treatment. A′, C′, Blots of total AKT after stripping the corresponding membrane with Thermo Scientific restore plus Western blot stripping buffer. Gray value analysis of each band was performed, and their ratios in correspondence to the total AKT band were calculated. The results demonstrated that 1 and 3 μm DHA significantly increase PTEN and decrease pAKT expression in cortical neurons **p < 0.001. ***p < 0.001. N = 3 or 4 independent samples.