Contralateral connections from the Mauthner cell to MiD cells in r5 and r6. An AP of the M-cell produced long-lasting depolarization in MiD cells on the contralateral side, whereas short hyperpolarization preceded the depolarization only in MiD2cm, with Erest of −80 mV for MiD2cm (A), −78 mV for MiD3cm (B), −83 mV for MiD2i (C), and −75 mV for MiD3i (D), respectively. Depolarizations evoked in MiD2i and MiD3i were similar in shape. The initial hyperpolarizing response observed in MiD2cm cell was changed in amplitude and reversed in polarity when the cell was polarized (−20, −15, −10, −5, +5, +8 nA; A, bottom). Red trace is the response at Erest. The calibration for top trace of A is applicable to B–D. E, F, Bar graphs quantifying PSP amplitudes and peak times from the onset at Erest; **p < 0.01, *p < 0.05; n.s., Not significant.