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. 2019 Oct 3;4(19):e129110. doi: 10.1172/jci.insight.129110

Figure 5. Kiaa0317 knockout confers protection from LPS-induced lung inflammation.

Figure 5

(A) sgRNA target site amplification from Kiaa0317+/+, Kiaa0317+/–, and Kiaa0317–/– mice. (B) Kiaa0317 transcript levels among Kiaa0317+/+, Kiaa0317+/–, and Kiaa0317–/– mice. Data represent mean values ± SEM (n = 3 mice). (C–G) Kiaa0317+/+, Kiaa0317+/–, and Kiaa0317–/– mice were i.t. inoculated with LPS (3 mg/kg) for 18 hours. Data represent mean values ± SEM (n = 3 mice per group). (C and D) Protein concentration and cell count from BALF. (E) BALF IL-6 concentration. (F) BALF TNF concentration. (G) BALF IL-1β concentration. (H) Histology of murine lungs following H&E staining; scale bar: 100 μm. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 compared with the indicated group or to sex-specific +/+ group; 1-way ANOVA with Tukey’s multiple-comparisons (B) or 1-way ANOVA with Dunnett’s multiple-comparisons test (C–G).