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. 2019 Oct 16;14(10):e0223775. doi: 10.1371/journal.pone.0223775

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© 2019 Quintana-Bustamante et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 3 — (A) Diagram of 5’UTR of of the Venezuelan Equine Encephalitis Virus (VEEV) (and 3’UTR β-Globin modifications added in PKLR TALEN^® subunits to synthesis in vitro mRNA. (B) Representative Surveyor assay of nucleofected CB-CD34⁺ cells with PKLR TALEN^® DNA plasmids or different doses of PKLR TALEN mRNA with at VEEV 5’, β-globin 3’ or at both UTRs at 3 days after nucleofection. (C) CFU analysis of nucleofected CB-CD34⁺ with PKLR TALEN^® DNA plasmids or different doses of mRNA. Kruskal-Wallis test was performed; P value is indicated in the figure. (n = 3; mean±SD).