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. 2019 Oct 16;14(10):e0223775. doi: 10.1371/journal.pone.0223775

Fig 6. Effect of dmPGE2 in the gene editing protocol.

Fig 6

(A) Representative FACS analysis of human engraftment 4 months post-transplant of NSG mice transplanted with nucleofected CB-CD34+ cells with PKLR TALEN® and donor matrix and cultured in presence of dmPGE2. (B) Nested PCR analysis of individual CFUs derived from puromycin resistant CB-CD34+ cells after PKLR TALEN® and donor matrix nucleofection. Presence or absence of dmPGE2 is indicated. The expected band size is marked with an arrow. λ DNA marker was used to identify the PCR band size.