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| 4d+2d protocol | CFU from PuroR cells | HR CFU | %HR |
|---|---|---|---|
| CD34-HR4 | 6 | 6 | 100 |
| CD34-HR5 | 21 | 15 | 71.4 |
| CD34-HR6 | 40 | 11 | 27.5 |
| CD34-HR7 | 21 | 15 | 71.4 |
| Mean | 67.6 | ||
| SEM | 15.0 | ||
Data from four independent experiments indicating the number of CFUs per 5000 PuroR cells, the number of CFUs positives for homologous recombination analysis and the percentage of gene edited CFUs. All the CFUs were derived from TM transfected and puromycin selected hematopoietic progenitors.
