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. 2019 Oct 16;14(10):e0223773. doi: 10.1371/journal.pone.0223773

Fig 1. Amplification and assembly of a scFv.

Fig 1

a Amplification of the light and heavy variable regions of mAb-10D8. Light variable portion amplification products are about 350 bp and heavy variable portion amplification products are about 400 bp. PCR products were obtained using cDNA as template. b Bidimensional representation of the scFv-10D8 (accession # MN106365) by IMGT/Collier de Perles highlighting the hypervariable regions (CDRs) of each variable chain. The amino acid residues were numbered according to the standard IMGT. Residues at positions 23, 41, 89, 104 and 118 are critical for the antibody structure and function. The coloured circles correspond to the CDRs of each chain, and the hatched circle indicates gaps that have been introduced for better alignment with the sequences of deposited variable regions.