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. 2019 Oct 16;14(10):e0223773. doi: 10.1371/journal.pone.0223773

Fig 4. Effect of pre-incubation of metacyclic trypomastigotes (MTs) with scFv-10D8 on LLC-MK2 cell invasion.

Fig 4

a MTs of T. cruzi G strain were pre-incubated with different dilutions of periplasmic fractions containing sFv-10D8 or an unrelated scFv for 2 h. b MTs of T. cruzi G strain were pre-incubated with different concentrations (250 and 2500 ng) for both purified protein fractions (scFv-10D8) and unrelated scFv (scFv Loxo) for 2 h and a negative control (NC, without antibody presence). FR.A and FR.B correspond to two independent fractions enriched for scFv-10D8. Statistically significant differences are represented by letters above the graph bar. The parasites were washed and incubated with LLC-MK2 cells at MOI 100:1. The percentage of infected cells was determined by the mean of blind counts of 300 cells from three independent experiments. The main statistically significant differences are indicated by horizontal bars above the columns (p ≤ 0.05).