Video 4. Lateral view of tracheal invasion into DVMs in control and tracheal-specific Mmp1 knock-down animals.

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DOI: 10.7554/eLife.48857.017

Time-lapse movies of tracheal invasion into DVMs in pupae 48 h APF. Tracheal cells are labeled by palmitoylated mKate2, IFMs are labeled by Myofilin-GFP. Lateral views of a wild-type control pupa (top) and a tracheal Mmp1 knock-down pupa (bottom) are shown. The movies were acquired with a 40x objective and a frame rate of 10 min in resonant scanning mode (Leica SP8) over 14 h. Z-stacks of ~100 μm (0.35 μm step size) were acquired.