Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Drosophila melanogaster) | mef2-Gal4 |
Ranganayakulu et al., 1995
Dev Biol, 171(1), 169–181 |
||
| Genetic reagent (Drosophila melanogaster) | btl-Gal4 | Shiga et al., 1996 Development, Growth and Differentation, 38(1), 99–106 | ||
| Genetic reagent (Drosophila melanogaster) | UAS-palmKate2-K7 | Caviglia et al., 2016 Nat Cell Biol 18: 727–739 | ||
| Genetic reagent (Drosophila melanogaster) | UAS-palm-mNeonGreen | Sauerwald et al., 2017Development 144: 657–663 | ||
| Genetic reagent (Drosophila melanogaster) | UAS-FRTFRT>STOP>FRT-myr::smGFP-HA_V5_FLAG | Nern et al., 2015 PNAS 112: E2967–76 | ||
| Genetic reagent (Drosophila melanogaster) | y1 v1;; UAS-Mmp1_RNAi_(1) | Bloomington Stock Center | BDSC: 31489 | |
| Genetic reagent (Drosophila melanogaster) | UAS-Mmp1_RNAi_(2) | Uhlirova and Bohmann, 2006 EMBO J 25(22): 5294–5304 | ||
| Genetic reagent (Drosophila melanogaster) |
;; FlyFos066598_
DpseMmp1_GA18484 (attP-9A/VK13) |
this study | Mmp1 ortholog GA18484 fromDrosophila pseudoobscura in genomic FlyFos vector inserted into VK13 attPlanding site (76A2).See “Drosophila strains and genetics “in theMaterials andmethods section. | |
| Genetic reagent (Drosophila melanogaster) | FRTG13 mmp1Q273/CyO, Dfd-GMR-YFP | this study | See “Genetic labeling of tracheal cell clones“in the Materials and methods section |
|
| Genetic reagent (Drosophila melanogaster) | FRTG13 mmp1Q112/CyO, Dfd-GMR-YFP | this study | See “Genetic labelingof tracheal cell clones “in the Materialsand methods section | |
| Genetic reagent (Drosophila melanogaster) | FRTG13 mmp12/CyO, Dfd-GMR-YFP | this study | See “Genetic labeling of tracheal cell clones “in the Materialsand methods section | |
| Genetic reagent (Drosophila melanogaster) | LanB1-GFP | Vienna Drosophila Resource Center; Sarov et al., 2016 | VDRC: fTRG681 | |
| Genetic reagent (Drosophila melanogaster) | Mmp1-GFP | Vienna Drosophila Resource Center; Sarov et al., 2016 | VDRC: fTRG145 | |
| Genetic reagent (Drosophila melanogaster) | Myofilin-GFP | Vienna Drosophila Resource Center; Sarov et al., 2016 | VDRC: fTRG501 | |
| Genetic reagent (Drosophila melanogaster) | Vkg-GFP | Buszczak et al., 2007 Genetics 175: 1505–1531 | ||
| Genetic reagent (Drosophila melanogaster) | UAS-TIMP | Bloomington Stock Center | BDSC: 58708 | |
| Genetic reagent (Drosophila melanogaster) | amph26 | Bloomington Stock Center | BDSC: 6498 | |
| Antibody | Rabbit polyclonal anti-Bnl | Jarecki et al., 1999 Cell, 99(2): 211–220 | 1:250 | |
| Antibody | Mouse monoclonal anti-Mmp1 | Developmental Studies Hybridoma Bank | Mix of mouse monoclonal antibodies 3B8D12, 3A6B4, 5H7B11 |
1:10/1:100/1:100 |
| Antibody | Mouse monoclonal anti-Dlg1 | Developmental Studies Hybridoma Bank | 4F3 | 1:200 |
| Antibody | Mouse monoclonal anti-DSRF | Samakovlis et al., 1996 Development 122(5): 1395–1407 | mAb 2–161 | 1:100 |
| Antibody | Mouse monoclonal anti-ATP5A | Abcam | ab14748 | 1:100 |
| Antibody | Chicken polyclonal anti-GFP | Abcam | ab13970 | 1:500 |
| Antibody | Chicken polyclonal anti-HA | Abcam | ab9111 | 1:200 |
| Antibody | Mouse monoclonal anti-FLAG (M2) | Sigma | M2; Sigma-F1804 | 1:1000 |
| Antibody | Guinea pig polyclonal anti-LaminDm0 | gift from Georg Krohne, University of Würzburg | 1:1000 | |
| Antibody | Rabbit polyclonal anti-Laminin | Schneider et al., 2006 Development 133(19):3805–3815 | 1:1500 | |
| Chemical compound, drug | Phalloidin-TRITC | Sigma | 1:1000 | |
| Recombinant DNA reagent | fosmid clone FlyFos066598 | Ejsmont et al., 2009 Nat Methods 6 (6): 435–437 | FlyFos066598 | fosmid clone FlyFos066598 containing the Drosophila pseudoobscura Mmp1 homologue GA18484 |